Despite their hard work, some mares still abort seemingly out of the blue, or give birth to a “peanut,” a small, runty foal which struggles to gain weight and thrive.

Unfortunately, any number of diseases or conditions can cause these outcomes, and it often feels like there is nothing we can do to prevent them.

Fortunately, there have been massive developments in research techniques recently that allow researchers to understand the underlying mechanisms of disease.

These range from genetic analyses to RNA-sequencing and advanced proteomics and lipidomics. In brief, DNA is transcribed into messenger RNA (mRNA), which can be translated into protein. Specific proteins (enzymes) alter the use and form of lipids, and each has a slightly different role in our understanding of health and disease.

Together, these techniques use the interconnected systems of genetic material and proteins to provide a more holistic understanding of normal and abnormal pregnancy and can be used to identify better ways to diagnose, treat, and even prevent pregnancy complications.

Genetic analysis provides information about a horse’s specific DNA. This remains the same throughout a horse’s life and can be passed directly to their offspring. Perhaps the best-known genetic testing in horses isthe 5-panel test in Quarter Horses, which includes tests for hyperkalemic periodic paralysis (HYPP), hereditary equine regional dermal asthenia (HERDA), polysaccharide storage myopathy (PSSM), and others.

If a breeder knows that their mare has one copy of any of these genes, they can avoid complications in their foals if they make sure they breed to a “clean” stallion,
or one who does not have a copy of that gene, which makes our horses healthier overall. Additionally, there is work being done to identify the genetic basis for umbilical cord torsion, placentitis, red bag delivery, and others.

RNA-sequencing analyses can assess the amount of mRNA produced in a tissue during disease. Because mRNA expression is the first thing to change during disease, we can often see the body’s immediate response to an infectious agent, as well as how that agent causes the body’s response to change over time. Additionally, mRNA is relatively simple to measure, allowing us to monitor expression of over 32,000 genes.

By monitoring this response, we can better understand what treatments are likely to help, what biomarkers may allow better diagnosis, and how to help prevent the horse from getting sick in the first place.

Lastly, proteins and lipids do the heavy lifting in tissues and are what exert the majority of the biological effects we see. Unfortunately, the technology we have in
place for testing large numbers of proteins and lipids is expensive and clumsy, making it difficult to look at changes which are happening at a cellular level.

Even so, proteins and lipids are often the best biomarkers because they remain in circulation for relatively long periods of time and reflect actual physiological changes
happening in response to the disease.

For example, proteomic studies have identified biomarkers for placentitis that change in blood and in amniotic fluid. From the outside, many of these molecular techniques seem pedantic and far removed from clinical reality; however, these techniques are what allow researchers to better understand the underpinnings of disease and how to diagnose and treat it.

RNA-sequencing has recently been used to identify how the mare and unborn foal act to fight both ascending and nocardioform placentitis,including how these diseases affect blood vessel development, nutrient transportation and immune signaling.

There have been other studies which have identified the key changes occurring in the placenta during early pregnancy – this work could ultimately lead to improved pregnancy diagnostic tests and better ways to assess pregnancy health.

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Press release by Equine Disease Quarterly April 2022 - Article by Shavahn Loux, PhD, Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky